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digital gel documentation system  (Thermo Fisher)


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    Structured Review

    Thermo Fisher digital gel documentation system
    Digital Gel Documentation System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/digital+gel+documentation+system/digital+gel+documentation+system/pmc11950904-118-81-86
    Average 90 stars, based on 1 article reviews
    digital gel documentation system - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Amplification:

    Article Title: B‐class gene GLOBOSA – a facilitator for enriched species diversity of Salvia in the New World?
    Article Snippet: The reaction volume was 30 μL, containing 20.4 μL nuclease‐free water (Lonza, Biozym), 1‐fold Thermopol Buffer (New England Biolabs), 1 mg mL −1 bovine serum albumin, 200 mM dNTPs (New England Biolabs), and 0.2 mM forward and reverse primers (see primer list, Table ), with 3 units Taq polymerase (New England Biolabs). .. Thermal cycler conditions for amplification of B‐class genes included initial denaturation at 95°C for 2 min; following 35 cycles at 94°C for 45 s, 58°C for 45 s, and 68°C for 2 min; ending with an extension of 68°C for 5 min. We subsequently evaluated the amplicons using agarose gel electrophoresis with NEEO ultra‐quality agarose (Carl Roth, Karlsruhe, Germany), visualizing DNA with SYBRsafe (Invitrogen, Thermo Fisher Scientific, Germany) or Midori green Xtra (Nippon Genetics Europe) upon blue light excitation on a digital gel documentation system (SafeImager; Invitrogen). .. The fragment size was determined using 100 bp or 1 kb size standards (New England Biolabs).

    Agarose Gel Electrophoresis:

    Article Title: B‐class gene GLOBOSA – a facilitator for enriched species diversity of Salvia in the New World?
    Article Snippet: The reaction volume was 30 μL, containing 20.4 μL nuclease‐free water (Lonza, Biozym), 1‐fold Thermopol Buffer (New England Biolabs), 1 mg mL −1 bovine serum albumin, 200 mM dNTPs (New England Biolabs), and 0.2 mM forward and reverse primers (see primer list, Table ), with 3 units Taq polymerase (New England Biolabs). .. Thermal cycler conditions for amplification of B‐class genes included initial denaturation at 95°C for 2 min; following 35 cycles at 94°C for 45 s, 58°C for 45 s, and 68°C for 2 min; ending with an extension of 68°C for 5 min. We subsequently evaluated the amplicons using agarose gel electrophoresis with NEEO ultra‐quality agarose (Carl Roth, Karlsruhe, Germany), visualizing DNA with SYBRsafe (Invitrogen, Thermo Fisher Scientific, Germany) or Midori green Xtra (Nippon Genetics Europe) upon blue light excitation on a digital gel documentation system (SafeImager; Invitrogen). .. The fragment size was determined using 100 bp or 1 kb size standards (New England Biolabs).



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